|
KMID : 0620919960280040181
|
|
Experimental & Molecular Medicine
1996 Volume.28 No. 4 p.181 ~ p.186
|
|
|
An enzyme immunoassay for pregnancy-specific β-1 glycoprotein (SP1), employing monoclonal antibodies
|
|
Jae Ho Lee/Jae Ho Lee
Seung Ryul Kim/Hong Keun Chung
|
|
|
Abstract
|
|
|
|
Two-site binding enzyme immunoassay for pregnancy-specific -β1 glycoprotein (SP1) has been developed using two monoclonal antibodies which can recognize all the three forms of SP1 from human sera. This assay system detects a minimal concentration of 1.25 ng/ml, and shows a linear dose-response curve at a range between 1.4 and 45.0 ng/ml. The recovery rates ranged from 90.0% to 113.3%, and intra-assay coefficients of variation were 15.8% and 8.7% for two different samples. When compared with a commercial EIA kit, this assay system had high correlation coefficient (r =0.92) but with somewhat lower values.
|
|
|
KEYWORD
|
|
|
enzyme immunoassay, monoclonal antibody, pregnancy-specific β-1 glycoprotein (SP1),
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|